5-Amino-1MQ is a small-molecule methylquinolinium reference compound used in laboratory studies of nicotinamide N-methyltransferase, methylation capacity, and NAD⁺-salvage pathway biology. It is not a peptide, and this page focuses on compound identity, assay design, and evidence limits for in-vitro research.
Small-molecule identity
Unlike the peptide materials in much of the Helix catalog, 5-Amino-1MQ is a small-molecule methylquinolinium iodide reference compound. The catalog record describes it as C₁₀H₁₁IN₂ with CAS 42464-96-0. A researcher should verify salt form, molecular weight convention, purity, and lot identity against the COA before preparing standards or comparing results between suppliers.
NNMT as a research target
Nicotinamide N-methyltransferase transfers a methyl group from S-adenosyl-L-methionine to nicotinamide, producing 1-methylnicotinamide and S-adenosyl-L-homocysteine. In laboratory research, an NNMT inhibitor tool compound can help investigators test how methyl-donor use, nicotinamide clearance, and methylation balance interact with cellular metabolism. Enzyme selectivity must be established in the actual assay rather than assumed from a product label.
Assay design
Useful experiments may include purified-enzyme kinetics, cellular target-engagement assays, metabolite measurements, and orthogonal confirmation of NNMT inhibition. Controls should account for solvent, cell permeability, incubation time, compound stability, and possible off-target effects. NAD⁺-related observations should be measured directly where possible, because changes in a downstream marker do not identify the causal pathway by themselves.
Research-only handling
Review the SDS and COA, use appropriate chemical hygiene, and document stock preparation and analytical conditions. This page is an educational summary of published research themes, not a protocol or a medical recommendation. The material is supplied strictly for laboratory Research Use Only and is not for human or veterinary use.
Every 5-Amino-1MQ order includes a sealed, lot-controlled vial, the completed lot documentation, and a handling & storage card. Product-specific handling follows the supplied documentation and institutional SOPs.
Evidence limits and research safety
Research findings can differ by model, assay, purity, formulation, and study design. Published cell, biochemical, and pre-clinical observations do not establish human or veterinary safety, efficacy, dosing, or suitability. Researchers should review the applicable SDS, institutional safety procedures, waste requirements, and lot-specific documentation before handling any reference material.
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