HELIX BioScience

Certificate of Analysis
Helix Reagents LLC

Orlando, FL
US · getpeptides.com
SPECIMEN — ILLUSTRATIVE FORMAT. This certificate shows the COA layout for this compound. Lot number, dates, and measured results (shown as “____”) are completed on the certificate issued with each shipped lot.

Product Identification

ProductTirzepatide (HXR-TIRZEPATIDE)
Full nameTirzepatide (dual GIP/GLP-1 receptor agonist peptide; LY3298176)
CAS No.2023788-19-2
Molecular formulaC₂₂₅H₃₄₈N₄₈O₆₈
Molecular weight4813.5 g/mol
AppearanceWhite lyophilized powder
Lot No.____
Manufacture date____
Re-test date____
Quantity30 mg

Analytical Results

TestMethodSpecificationResult
PurityRP-HPLC≥98% (HPLC)____
IdentityESI-MSConsistent with MW____
AppearanceVisualWhite powder____
SolubilityVisualSoluble in water / AcOH____
Water contentKarl Fischer≤ spec____
Counterion (acetate / TFA)HPLCReport____

Analytical Spectra

RP-HPLC Chromatogram
Absorbance (220 nm) Retention Time (min) Tirzepatide
Column: C18, 4.6×150mm · Mobile phase: ACN/H₂O (0.1% TFA) · Flow: 1.0 mL/min
ESI Mass Spectrum
Relative Abundance m/z [M+3H]³⁺ [M+4H]⁴⁺ [M+5H]⁵⁺ calc. calc.
Ionization: ESI+ · Mass range: 200–2000 m/z · Deconvoluted MW consistent with theoretical
SequenceY-Aib-EGTFTSDYSI-Aib-LDKIAQKAFVQWLIAGGPSSGAPPPS (39-residue peptide; Aib at positions 2 and 13; C-terminal amide; Lys20 conjugated to a C20 fatty diacid via a γGlu–(AEEA)₂ linker)

Storage & Handling

Store at −20°C, desiccated, protect from light. Minimize freeze–thaw cycles. Reconstitute under aseptic laboratory conditions.
Reviewed / Approved by ____________________ (QC)
Date ____________________
RESEARCH USE ONLY
Not for human or veterinary use, food, drug, or therapeutic application.
Helix Reagents LLC · getpeptides.com ·
HXR-TIRZEPATIDE This certificate is valid only for the lot identified above.