HELIX BioScience

Certificate of Analysis
Helix Reagents LLC

Orlando, FL
US · getpeptides.com
SPECIMEN — ILLUSTRATIVE FORMAT. This certificate shows the COA layout for this compound. Lot number, dates, and measured results (shown as “____”) are completed on the certificate issued with each shipped lot.

Product Identification

ProductTB-500 (HXR-TB-500)
Full nameTB-500 (synthetic thymosin beta-4 fragment 17-23, acetylated)
CAS No.885340-08-9
Molecular formulaC₃₈H₆₈N₁₀O₁₄
Molecular weight889.0 g/mol
AppearanceWhite lyophilized powder
Lot No.____
Manufacture date____
Re-test date____
Quantity5 mg

Analytical Results

TestMethodSpecificationResult
PurityRP-HPLC≥98% (HPLC)____
IdentityESI-MSConsistent with MW____
AppearanceVisualWhite powder____
SolubilityVisualSoluble in water / AcOH____
Water contentKarl Fischer≤ spec____
Counterion (acetate / TFA)HPLCReport____

Analytical Spectra

RP-HPLC Chromatogram
Absorbance (220 nm) Retention Time (min) TB-500
Column: C18, 4.6×150mm · Mobile phase: ACN/H₂O (0.1% TFA) · Flow: 1.0 mL/min
ESI Mass Spectrum
Relative Abundance m/z [M+3H]³⁺ [M+4H]⁴⁺ [M+5H]⁵⁺ calc. calc.
Ionization: ESI+ · Mass range: 200–2000 m/z · Deconvoluted MW consistent with theoretical
SequenceAc-Leu-Lys-Lys-Thr-Glu-Thr-Gln (Ac-LKKTETQ; acetylated 7-residue fragment corresponding to residues 17-23 of thymosin beta-4 — distinct from full-length thymosin beta-4, CAS 77591-33-4)

Storage & Handling

Store at −20°C, desiccated, protect from light. Minimize freeze–thaw cycles. Reconstitute under aseptic laboratory conditions.
Reviewed / Approved by ____________________ (QC)
Date ____________________
RESEARCH USE ONLY
Not for human or veterinary use, food, drug, or therapeutic application.
Helix Reagents LLC · getpeptides.com ·
HXR-TB-500 This certificate is valid only for the lot identified above.